Coding

Part:BBa_M45155

Designed by: Logan Sherman   Group: UtahState_BE5930_-_S14   (2016-04-20)


M. tuberculosis antigen

This is a coding region for the Mycobacterium tuberculosis antigen MPT32. The sequence was back translated from the amino acid sequence and optimized for production in Escherichia coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 870
    Illegal AgeI site found at 327
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 627


[edit]
Categories
Parameters
None